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KMID : 0861020170320020107
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Korea Journal of Herbology
2017 Volume.32 No. 2 p.107 ~ p.114
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Effects of Tribuli Fructus extract on inflammatory responses in IgE-stimulated RBL-2H3 mast cells
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Roh Hyo-Sun
Park Yong-Kee
Bae Hyo-Sang
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Abstract
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Objectives : Tribulus terrestris Linne (Tribuli Fructus; TF) has been used to treat hypochondrium, agalactia, nebula, itching and vitiligo in traditional Korean medicine. In this study, we investigated the effects of TF 30% ethanol extract on inflammatory responses in IgE-stimulated RBL-2H3 mast cells.
Methods : TF extract was prepared by 30% ethanol. RBL-2H3 cells, a rat mast cell line, were treated with TF extract at different concentrations for 1 hr and then stimulated with DNP-IgE/HSA for indicated times. Cell viability was measured by WST-1 assay. The expression of inflammatory cytokines (IL-4, IL-13 and IFN-γ) mRNA was determined by reverse transcriptase-PCR, and the phosphorylation of ERK1/2, p38 and JNK MAP kinases (MAPKs) was determined by Western blot. The nuclear expression of NF-κB p65 in the cells was detected by Western blot and immunocytochemistry, respectively.
Results : The treatment of TF extract at 0.1 and 0.2 ㎎/㎖ significantly decreased the expression of IL-4 and IL-13 mRNA in IgE-stimulated RBL-2H3 mast cells, while significantly increased the expression of IFN-γ mRNA. TF extract treatment was also inhibited the phosphorylation of ERK1/2, p38 and JNK MAPKs in IgE-stimulated RBL-2H3 mast cells in a dose-dependent manner. In addition, TF extract significantly blocked the translocation of NF-κB p65 into the nuclear of cells after IgE stimulation.
Conclusions : These results indicate that TF extract inhibits inflammatory response in IgE-stimulated mast cells through blocking MAPKs/NF-κB pathway. This suggests that TF extract has an anti-inflammatory activity in mast cell activation.
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KEYWORD
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Tribulus terrestris Linne, Tribuli Fructus, RBL-2H3 cells, inflammatory responses, MAPKs/NF-κB pathway
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